Antibodies and chemicals

Anti-RSK1 (#9333S) antibody was ordered from Cell Signaling Technology. HRP anti-HA (#901519), HRP anti-Flag (#637311) antibodies were purchased from BioLegend. pRSK1 (Thr359/Ser363) (#AP0539), SUMO1 (#A19121), SUMO2/3 (#A2571), and ubc9 (#A4396) were purchased from ABclonal. Mouse anti-GFP (#sc-9996) antibody was ordered from Santa Cruz Biotechnology. Anti-RTA (ORF50) monoclonal mouse antibody was given by Dr. Ke Lan (Wuhan University, China) [26]. Anti-ORF65 monoclonal mouse antibody was given by Dr. Shou-Jiang Gao (University of Pittsburgh, USA) [27]. Monoclonal antibodies against ORF45 and ORF52 and polyclonal antibodies against K3 and K8 were described previously [10,28,29]. EZview Red Anti-Flag M2 Affinity Gel (#F2426), TPA (#P1585) and sodium butyrate (#B5887) were ordered from Sigma, Doxycycline (#S4163) was ordered from Selleck, Glutathione Sepharose 4B (#17-0756-01) was ordered from GE Healthcare. ClonExpress II One Step Cloning Kit (#C122-01) and HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (#R223-01) were purchased from Vazyme Biotech. FuGENE HD Transfection Reagent (#E2311) was ordered from Promega. Lipofectamine 3000 (#3000015) were purchased from Thermo Fisher Scientific.