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Histological evaluation

XZ Xianyu Zhou
XL Xusong Luo
BG Bowen Gao
FL Fei Liu
CG Chuan Gu
QY Qingxiong Yu
QL Qingfeng Li
HZ Hainan Zhu
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After Microfil angiography on POD 60, tissue specimens were harvested from all recipients for histological evaluation. Samples of the composite tissue components of forelimb were harvested, fixed in 10% formalin solution for 3 days, and then decalcified in Immunocal solution (Decal Chemical Corporation, Tallman, NY) for 14 days. Fixed specimens from mid-forelimb, wrist, and forepaw were then embedded in paraffin and cut into 7-μm cross-sections. Sections were then stained with hematoxylin-eosin and evaluated for signs of ischemia or any other pathological changes.

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