Full-field laser perfusion imager (FLPI) features of amputated limbs

Xianyu Zhou; Xusong Luo; Bowen Gao; Fei Liu; Chuan Gu; Qingxiong Yu; Qingfeng Li; Hainan Zhu

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Full-field laser perfusion imager (FLPI) features of amputated limbs

XZ Xianyu Zhou

XL Xusong Luo

BG Bowen Gao

FL Fei Liu

CG Chuan Gu

QY Qingxiong Yu

QL Qingfeng Li

HZ Hainan Zhu

This method is extracted from research article: Sci Rep, May 2017

A series of rat segmental forelimb ectopic implantation models

DOI: 10.1038/s41598-017-01342-x

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A full-field laser perfusion imager (FLPI, Moor instrument, UK) utilizes a 780 nm laser beam to measure the average blood speed in tissue and the concentration of traveling red blood cells in a tissue sample volume by performing contrast analysis on images acquired from a Charge-coupled Device (CCD) video camera. All measurements were performed in a warm (24 °C) and quiet environment. The CCD camera was positioned 30 cm above the tissue surface of each rat; settings for low-resolution/high-speed images included a display rate of 25 Hz, time constant of 1.0 s and camera exposure time of 20 ms. The height and system parameters remained constant in every case with values expressed in BPU. The contrast images were processed to produce a color-coded live flux image (red denoted high perfusion, blue signified low perfusion). The measurements were performed on POD 30.

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