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Cells at 2.5 × 103 were seeded in 0.3% low melting soft agar laid on 0.6% low melting soft agar support for 3-week incubation. Colonies were fixed with methanol and stained with 0.1% crystal violet in 20% methanol for 30 min. Each assay was performed in triplicate.
Copyright and License information: ©2022 Liu, Wang, Yang, Hu, Fu, Zhang and Li
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