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Mapping the DNMT3A-lncRNA interactome by RIP

YW Yichen Wang
LJ Lin Jia
CW Cong Wang
ZD Zhonghua Du
SZ Shilin Zhang
LZ Lei Zhou
XW Xue Wen
HL Hui Li
HC Huiling Chen
YN Yuanyuan Nie
DL Dan Li
SL Shanshan Liu
DF Daniela Salgado Figueroa
FA Ferhat Ay
WX Wei Xu
SZ Songling Zhang
WL Wei Li
JC Jiuwei Cui
AH Andrew R. Hoffman
HG Hui Guo
JH Ji-Fan Hu
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Fibroblasts were collected for immunoprecipitation. In order to map the DNMT3A-lncRNA interactome, the biotinylated lncRNA-cDNA/Dnmt3a complex was immunoprecipitated with anti-DNMT3A antibodies (ab88470; Abcam). The biotinylated lncRNA–cDNAs were further purified from genomic DNAs using M-280 streptavidin beads (Invitrogen; Zhang et al., 2014b). After purification, the DNMT3A-interacting lncRNA–cDNAs were quantitated by PCR using target gene primers.

Copyright and License information:  ©2022 Wang et al.
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