Calibration Curves, Accuracy and Precision

Three different DBS assay batches consisting of a zero blank, six levels of calibration standards (n = 2 per level), and QCs (n = 6 per QC level) were run. Calibration curves were constructed using a linear regression equation of analyte/IS peak area ratio against the nominal concentration. Accuracy was defined as the percentage deviation of the measured concentration from the nominal value, and precision was defined as the percentage coefficient of variation (%CV). For every validation or clinical sample analysis batch, not less than 75% of all standards and 67% of all QCs were required to have a percentage deviation within ±15%.