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Flow cytometry was performed as previously described [45]. Single-cell suspensions were prepared from liver NPCs and stained with the following directly labeled mouse-specific mAbs, Percp/Cy5.5-labeled anti-CD11b (clone M1/70) and PE-labeled anti-F4/80 (clone BM8). Antibodies were purchased from Biolegend and used at a 0.2 μg/ml concentration. Cells were collected on a FACS Calibur (BD Biosciences, San Diego, CA, USA) and analyzed by FlowJo software (TreeStar, Ashland, OR, USA).
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