2.21. In vivo treatment

Tumor-bearing C57BL/6 mouse were randomly separated into five groups (n = 5 per group), including G1: PBS, G2: PBS + Laser, G3: GSM + Laser, G4: GSMM, G5: GSMM + Laser. After 4 h injected with GSMM (5 mg/kg based on Au), GSM (5 mg/kg based on Au) or PBS, the mice were treated with a 1064 nm laser (0.6 W/cm²) for 10 min in G2, 3 and 5. The real-time temperature in tumor was recorded using infrared thermal imager (Fotric, Beijing, China). All mice were treated every five days. The body weights and tumor volume of mice were monitored every two days for ten days. Whereafter, the mice injected euthanasia and the tumors were dissected. Partial tumor specimens were fixed in 4% neutral buffered formalin, processed routinely into paraffin and sectioned at 4 μm. Then, the tumor sections were stained with Ki67 and Calreticulin (CST). Partial tumor tissues were used for following western blotting and flow cytometry.