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Retrograde labelling of RGCs with the fluorescent tracer

MS Masatoshi Sato
ES Eriko Sugano
KT Kitako Tabata
KS Kei Sannohe
YW Yoshito Watanabe
TO Taku Ozaki
MT Makoto Tamai
HT Hiroshi Tomita
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For RGC retrograde labelling, we used one-year-old Thy1.2 TG (TG-ChR2) rats subjected to photoreceptor degeneration into which the mVChR1-Cherry gene had been transduced instead of the mVChR1-Venus gene in the same manner as described above. To examine the transduction efficiencies of mVChR1 genes into RGCs, RGCs were retrograde-labelled with a fluorescent tracer by slowly injecting 4 μL of 2% aqueous fluorogold (Fluoro-Gold; Fluorochrome, Englewood, CO) containing 1% dimethyl sulfoxide into the superior colliculus using a Hamilton syringe with a 32-gauge needle.

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