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MALDI TOF/TOF of some protein bands.
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The peptides generated from the in-gel digestion were used to spot on the MALDI target plate. Initially, 2 μL of the peptide sample was spotted and allowed to dry before spotting 2 μL of HCCA matrix. The MALDI plate was then inserted into an MS instrument obtained from Bruker Daltonics (Bremen, Germany) and the acquired MS and MS/MS spectra of different peptides. MS data were acquired from 800 to 4,000 m/z. Proteins were identified using the MASCOT program.
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