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A fluorometric caspase 3 assay was used to determine the level of caspase 3 activations within samples. Samples were prepared following the manufacturer’s instructions. A Cytofluor 4000 spectrometer plate reader was used to determine fluorescence at an excitation wavelength of 450 nm and an emission wavelength of 530 nm. Statistical analysis was done by ANOVAs followed by Tukey's multiple-comparison test using GraphPad Prism 9 software and error bars represent standard deviation from the mean.
Copyright and License information: ©2022 Stonedahl et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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