Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
HCC xenograft models were created using HepG2 cells. Briefly, 1 × 107 cells were injected subcutaneously into the right flank of 6-week-old BALB/c nude mice (Orienbio, Seongnam, Korea). The tumor was allowed to grow till 100–200 mm3 (measured with a Vernier caliper). The tumor volume was calculated using the formula: V(volume) = L(length) × W(width) × H(height)/2. The mice were monitored weekly for tumor growth and GPC3_3 aptaprobe using in vivo imaging system (IVIS) imaging as described below.
Copyright and License information: The Author(s) ©2022
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
