Flow cytometry analysis

PBMCs incubated with or without CpG ODN (3 μg/mL) for 12 h and cells from abdominal lavage fluid were washed twice with FACS staining buffer (PBS supplemented with 0.5% BSA, 0.01% NaN₃ and 100 mM EDTA) and then analyzed for the expression of various cell surface molecules by staining with FITC-labeled anti-CD80 mAbs and CD4, CD8a, NK1.1 mAb (used at 1:100 dilution) respectively followed by analysis of FACSCalibur^34,35.