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Spinal cord cells were evaluated histologically using TEM. Mouse tissues were fixed and embedded following previously described methods (Pan et al., 2013b). Then, 70 nm thick slices were obtained and were attached to copper grids, stained with 1% uranium acetate and 1% lead citrate (both Sigma-Aldrich, St. Louis, MO, United States), and then observed using a JEM-1230 transmission electron microscope (JEOL Ltd., Tokyo, Japan).
Copyright and License information: ©2022 Gong, Zhu, Liao, Wang, Zheng, Wang, Liu and Pan.
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