Transient Transformation

The plasmid TRV2-SlERF.D6, TRV2 and TRV1 were introduced into A. tumefaciens strain, then injected into MG fruit with the TRV1 vector combined with TRV2 or TRV2-SlERF.D6 plasmid, the TRV2 vector was used as control. The injection was performed as described previously (Orzaez et al., 2006; Fantini and Giuliano, 2016). The skin samples were collected 10 days after injection and immediately placed in liquid nitrogen. The vector pBin18-E8-SlERF.D6 used for transient overexpression was introduced into Agrobacterium and then injected into the tomato MG fruit in the same way, as the pBin18-E8 vector was used as control (Orzaez and Granell, 2009; Zhao and Zhao, 2009). Samples (skin) were taken into liquid nitrogen after 8 days.