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Hematoxylin and eosin (H&E) staining was performed on 4 μm sections of formalin-fixed, paraffin-embedded breast cancer tissue from each MMR variant carrier. The staining procedures were as follows: dewaxing, dehydration, hematoxylin, differentiation, bluing, eosin, dehydration, clearing, and cover-slipping. The pathologists histopathologically evaluated the tumor cell area in each H&E section.
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