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Statistical analysis

MB Marie-Clotilde Bernard
FB Florence Boudet
AP Andrea-Clemencia Pineda-Peña
FG Françoise Guinet-Morlot
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Titers induced by vaccine immunization were statistically compared using a mixed model with product (PVRV-NG, PVRV, or HDCV alone or co-injected with hRIG or eRIG) and time as fixed factors and their interactions.

For group comparison (rabies vaccine + RIG versus rabies vaccine alone), a Tukey’s adjustment for multiple testing was performed. Only significant titer differences (i.e., titer decrease ≥ twofold) were considered biologically relevant considering the precision of the techniques.

A margin of error of 5% was used for effects of the main factors and of 10% for the interaction. The residuals of the model were studied to test the validity (normality, extreme individuals, etc.) of the model.

No replacement of missing or invalid values was made, and no imputation was performed for any of the analyses. A p-value < 0.05 was considered to indicate a statistically significant difference.

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