Prostate cancer cell culture

MT Martina Troiani
MC Manuel Colucci
MD Mariantonietta D’Ambrosio
IG Ilaria Guccini
EP Emiliano Pasquini
AV Angelica Varesi
AV Aurora Valdata
SM Simone Mosole
AR Ajinkya Revandkar
GA Giuseppe Attanasio
AR Andrea Rinaldi
AR Anna Rinaldi
MB Marco Bolis
PC Pietro Cippà
AA Andrea Alimonti
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PC3 and LNCaP human prostate cancer cells were purchased from ATCC (Cat. N CRL-1435 and CRL-1740 respectively) and were cultured according to the manufacturer’s instructions. Cells were cultured in RPMI 1640 supplemented with 10% FBS and 1% P/S. HEK-293T (human embryonic kidneys, Cat. N CRL-3216) and TrampC1 (Cat. N CRL-2730) cells were obtained from ATCC. RapidCap were obtained from Trottman laboratory63. Cells were cultured in DMEM supplemented with 10% FBS and 1% P/S. All cell lines were kept under controlled temperature (37 °C) and CO2 (5%) and used for experiments at early passages. All the cell lines were tested negative for Mycoplasma (MycoAlertTM mycoplasma detection kit, LT07-418, LONZA).

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