PC3 and LNCaP human prostate cancer cells were purchased from ATCC (Cat. N CRL-1435™ and CRL-1740™ respectively) and were cultured according to the manufacturer’s instructions. Cells were cultured in RPMI 1640 supplemented with 10% FBS and 1% P/S. HEK-293T (human embryonic kidneys, Cat. N CRL-3216™) and TrampC1 (Cat. N CRL-2730™) cells were obtained from ATCC. RapidCap were obtained from Trottman laboratory63. Cells were cultured in DMEM supplemented with 10% FBS and 1% P/S. All cell lines were kept under controlled temperature (37 °C) and CO2 (5%) and used for experiments at early passages. All the cell lines were tested negative for Mycoplasma (MycoAlertTM mycoplasma detection kit, LT07-418, LONZA).
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