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The QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany) and the QIAamp DNA FFPE Tissue Kit (Qiagen, UK) were used to extract normal DNA from blood and tumour DNA from FFPE tumour samples, respectively, according to the manufacturer’s instructions. The Qubit 2.0 fluorometer and the Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, USA) were used to measure DNA concentration.
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