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Tumour tissue was collected from formalin-fixed, paraffin-embedded blocks of the primary tumours from 1004 of the original 1178 randomised patients. The material includes 140 surgically treated IBTRs from patients with a primary tumour available in the TMA for matching. TMA construction was in a semi-automated TMA arrayer (Pathology Devices, Westminster, MD) by extraction of two 1.0-mm cylinders from representative tissue from each tumour block.
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