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2.2.3. Determination of Serum Inflammatory Factors
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All the subjects collected venous blood samples (5 ml) on an empty stomach. Within 1 hour, the specimens were centrifuged, 3000 r/min and centrifuged 10 min, and the serum was separated. After subpackaging, it was stored at-20°C. IL-4, IL-6, IL-10, TNF-α, TNF-β, CCL-2, and enzyme-linked antibody adsorption test (ELASIA) kits were employed in Shanghai Langton Biotechnology Co., Ltd., and all samples were examined by ELASIA kit after collection.
Copyright and License information: ©2022 Yan Lu et al.
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