Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Physicochemical parameter analysis

TS Tushar Ahmed Shishir
TJ Taslimun Jannat
IN Iftekhar Bin Naser
request Request a Protocol
ask Ask a question
Favorite

The spike protein of the reference and Omicron variants were subjected to preliminary sequence analyses to distinguish their physicochemical differences. The amino acid composition, molecular weight, distribution of charged residues, hydropathicity, aliphatic index, instability index, and a few other parameters are calculated mainly using the online server EMBOSS Pepstats [29]. Further verification of the results from Pepstats was conducted using ProtParam, Prosite and AA-prop [32, 33]. In addition, another web server called VOLPES was used to compare and visualize residue-level physicochemical properties [34].

Copyright and License information:  ©2022 Shishir et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A