Mitochondrial Division Inhibitor and STAT3 Inhibitor

Mdivi-1 (338967-87-6, Selleck, Shanghai, China), a mitochondrial division inhibitor, is a highly efficient small molecule that selectively inhibits the activity of Drp 1 GTPase by blocking the self-assembly of Drp1 (24). Mdivi-1 was formulated as 10 mM liquid storage with dimethylsulfoxide, and was used to inhibit Drp1 at a concentration of 10 μM. HO-3867(HY-100453, MCE, USA), an analog of curcumin, is a selective STAT3 inhibitor that inhibits STAT3 phosphorylation, transcription, and DNA binding activity without affecting the expression of other active STATs. HO-3867 was formulated as 10 mM liquid storage with dimethylsulfoxide, and was used to inhibit STAT3 at a concentration of 10 μM (25). Cryptotanshinone(Cry) (HY-N0174, MCE) is a STAT3 inhibitor, that strongly inhibits the phosphorylation of STAT3 Tyr705, and has a weak effect on STAT3 Ser727. Cry was formulated as 10 mM liquid storage with dimethylsulfoxide, and was used to inhibit STAT3 at a concentration of 7 μM (26).