Animal Treatments

All animal experiments were approved by the Ethics Committee of the Second Military Medical University (Shanghai, China). A total of 32 of male Sprague-Dawley rats (weighing 180–220 g) were obtained from Shanghai Sippr-BK laboratory animal Co. Ltd. (SCXK2013-0016). Under appropriate temperature (22 ± 2°C) and humidity (55 ± 5%), animals were adapted to the new experimental environment (12 h light-dark cycle) for 1 week. After that, the rats were divided into four groups with eight rats in each randomly: control (CON), model (CUMS), zhi-zi-chi decoction (ZZCD), and fluoxetine (Flu). The Flu group served as the positive group. For the next 7 weeks ( Zhao et al., 2020 ), all the rats were fed alone and followed the CUMS design except CON group (Supplementary Tables S1, S2). For the last 2 weeks, the rats in ZZCD group were given 5 g raw herb/kg ZZCD. In addition, the rats in Flu group were given 10 mg/kg fluoxetine, and the rats in CON and CUMS group were given CMC-Na solution. ZZCD and fluoxetine were dissolved in 0.5% CMC-Na solution. The bodyweight of all rats was recorded on day 1, 20, 36, and 52.