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Variant Confirmation

CV Clara Vicente-Garcés
EE Elena Esperanza-Cebollada
SM Sara Montesdeoca
MT Montserrat Torrebadell
SR Susana Rives
JD José Luis Dapena
AC Albert Català
NC Nuria Conde
MC Mireia Camós
NV Nerea Vega-García
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Mutations found by NGS with VAF>15% were confirmed by Sanger sequencing and fusions detected by the panel were confirmed by RT-qPCR, using ABL1 as a housekeeping gene. Different and specific primer sets were designed using PRIMER3plus software (https://primer3plus.com/cgi-bin/dev/primer3plus.cgi). For fusions, 500 ng of total RNA was retrotranscribed with the High Capacity Retrotranscription kit (Applied Biosystems).

To confirm the somatic or germline nature of the variants, we used patient-matched bone marrow samples in complete morphological remission (CR) with negative measurable residual disease (MRD), as assessed by 8-color flow cytometry.

Copyright and License information:  ©2022 Vicente-Garcés, Esperanza-Cebollada, Montesdeoca, Torrebadell, Rives, Dapena, Català, Conde, Camós and Vega-García.
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