Collection information

In June 2018, T. lepidozioides was collected using aseptic methods, and under permit, by G. K. Golinski from Takakia Lake, Moresby Island, Haida Gwaii, British Columbia, Canada (52°55′48″N, 132°04′15″W). At this coastal northern temperate rain forest site, the average high temperature during the month of collection is 10°C, and the average low temperature 4°C. A fist-sized clump dominated by this moss that included a few thalloid liverworts and angiosperm seedlings but not soil, was stored over damp paper towels in a clear plastic box (allowing light penetration) in a lighted refrigerator in the University of British Columbia Herbarium for a month prior to cleaning and processing for subsequent DNA extraction or microscopy. This storage regime might have influenced the relative abundances of microbes indicated by DNA extracted a month later, but was designed to reduce the chances of microbial contamination or overgrowth during this period, and by maintaining conditions conducive for plant survival, to reduce the chances for loss of key microbial associates.