Short-term colony-forming cell (CFC) assay

JY Jin-Song Yan
MY Meng-Ying Yang
XZ Xue-Hong Zhang
CL Chen-Hui Luo
CD Cheng-Kan Du
YJ Yue Jiang
XD Xuan-Jia Dong
ZW Zhang-Man Wang
LY Li-Xue Yang
YL Yi-Dong Li
LX Li Xia
YL Ying Lu
ask Ask a question
Favorite

Purified primary CD34+ cells were cultured in Stemspan serum-free medium (Stem Cell Technologies) containing 10 ng/mL human SCF, and 10 ng/mL human TPO (Peprotech) in the presence of metformin. Forty-eight hours after the culture, the cells were transferred to a methylcellulose-based medium (Methocult H4034 Optimum, StemCell Technologies) in triplicate, and colonies were manually counted after 10–14 days.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A