Chemicals

Bovine thyroglobulin (BTG), porcine pepsin (P-6887) and trypsin from Porcine Pancreas (Proteomic Grade, BioReagent, Dimethylated) were obtained from Sigma-Aldrich (St. Louis, MO, USA). HRP-conjugated mouse anti-human IgE (B3102E8, Abcam, UK), goat anti-human IgE (KPL, Gaithersburg, MD, USA), monoclonal mouse anti-α-Gal (M86, Enzo Life Science Inc, Farmingdale, NY, USA), AP-conjugated goat anti-mouse IgM (Southern Biotech, Birmingham, AL, USA) as well as FITC conjugated anti-CD63 and PE conjugated anti-CD203c (clones CLBGran/12 and 97A6, respectively, Immunotech, Marseille, France) antibodies were purchased. PNGase F kit for deglycosylation was obtained from New England BioLabs (Ispwich, MA, USA). HSA-NAc-β-1,4-galactose, HSA-NAc-β-1,4-galactose-α-1,3-galactose and HSA- galactose-α-1,3-galactose were obtained from Dextra Laboratories, Reading, UK. Deionized water for all experiments was purified in a Milli-Q system (Millipore, Molsheim, France). All other reagents (analytical grade) were purchased from Sigma-Aldrich.