Atractylodin with purity of 98% was obtained from Chengdu Mustbio Technology co., Ltd., China. MLCK antibody and GAPDH antibody were purchased from Abcam (Cambridge, UK). Tumor necrosis factor-alpha (TNF-α), Interleukin-1-beta (IL-1β), and Interleukin-6 (IL-6) double-antibody sandwich ELISAs were purchased from Sigma (St. Louis, MO, USA). Anti-rabbit IgG secondary antibody was bought from Cell Signaling Technology (CST, Beverly, MA, USA). 4,6-diamidino-2-phenylindole (DAPI) was provided by Sigma (St. Louis, MO, USA).
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