Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Male adult rats (8 weeks, 180~220 g) were randomized by weight and anesthetized with isoflurane and subjected to intra-articular injection with 1.5 mg of MSU crystals dissolved in 50 μl PBS in one ankle joint. PBS only was given to the contralateral ankle as a control. Intra-articular administration of forskolin (30 μM), bucladesine (300 μM), SQ22536 (100 μM), and H-89 (10 μM) was given to animals 30 min earlier before MSU injection. Ankle swelling was tested using a Vernier caliper.
Copyright and License information: ©2022 Liu, Zhou, Jiang, Ye, Tian, Jiang, Hao, Li and Hu
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
