3.7. Antitrypanosomal, Antileishmanial, Antiplasmodial, and Cytotoxicity Assays

The in vitro activity was assessed on T. b. rhodesiense (STIB900, bloodstream forms), T. cruzi (Tulahuen C2C4, intracellular amastigotes), L. donovani (MHOM-ET-67/L82, axenically grown amastigotes), P. falciparum (NF54, intraerythrocytic), and L6 cells (rat skeletal myoblasts) as previously described [42]. Results are expressed in µg/mL for extracts and in µM for pure compounds. Samples were considered active when their IC₅₀ values were <50 µg/mL for extracts and <50 µM for pure compounds.