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The ARS-853 stock solution (Glpbio, USA) was diluted to a concentration of 25 μM in the cell culture medium (Gibco, USA). DMSO (Sigma-aldrich, USA) alone was dissolved as negative (vehicle) control. After that, western blotting, cell proliferation and cell cycle assays were performed after incubation with or without the Ras signaling pathway inhibitor ARS-853 (25 nM) for an appropriate period.
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