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Targeted amplicon sequencing and data analysis
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To prepare the targeted amplicon library, gDNA was extracted from the cells and further amplified using DNA primers (Additional file 1: Table S2). Nested PCR (denaturation at 98°C for 30 s, primer annealing at 62°C for 15 s, and elongation at 72 °C for 15 s, 35 cycles) was performed to conjugate adapter and index sequences to the amplicons. All targeted amplicon sequencing and data analysis were performed as suggested in a previous study [23].
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