Magnetic Bead Immunoassay and Peritoneal Analyte Quantification

At necropsy, 1mL of PBS buffer was carefully injected and retracted from the cavity of naïve, cured and reinfected mice using a 20G needle and syringe. These peritoneal exudate samples were then centrifuged, the supernatants were collected and stored at -20°C for performing a multiplex sandwich ELISA-based Luminex immunoassay using the Mouse Cytokine & Chemokine 26-plex ProcartaPlex kit as per the manufacturer’s instructions. The results were read using a Luminex MagPix machine. The data presented for this assay are pooled from two independent experiments with 3-4 mice per group in each experiment.