The statistical analysis was conducted by the Research Support Unit of the IT Department of the Complutense University of Madrid using the SPSS 27, v9.4 statistical package (SAS Institute, Cary, NC, USA). Calibration curves were created using Excel software (Microsoft Office 365) and all graphs were designed with GraphPad Prism 8 software (GraphPad Software, La Jolla, CA, USA). Normal distribution of data was determined using the Kolmogorov-Smirnov test. As regards individual measurements, sex differences were evaluated using Student's t-test. Results were considered statistically significant if p-value < 0.05.
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