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Control and clodronate liposomes were purchased from Liposoma Research. The right flank was shaved, and 50 µl of control liposomes (containing PBS) (PBS-L) or clodronate liposomes (CLL) was intradermally injected 7 days prior to tumor implantation. Liposomes were then injected weekly to maintain macrophage depletion. For systemic CLL treatment, 200 µl of control PBS-L or CLL were injected intraperitoneally (i.p.) 7 days prior to tumor implantation and then weekly to maintain macrophage depletion.
Copyright and License information: ©2022 Louie, Oo, Leung, Lin, Stephens, Alrashed, Tso and Liao.
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