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2.3. Total RNA extraction and cDNA synthesis

EK Elham Karimi
HA Hanieh Azari
AT Ahmad Tahmasebi
AN Amin Reza Nikpoor
AN Ahmad Agha Negahi
NS Nima Sanadgol
MS Mohammad Shekari
PM Pegah Mousavi
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Extraction of total RNA was performed using the triazole method according to the protocol of the RNX‐PLUS kit (Sinaclon). Quantification of extracted RNAs was evaluated using Nanodrop (Thermo Scientific™), and RNA quality was assessed by electrophoresis on 2% agarose gels. The cDNA synthesis kit (TAKARA) was used to convert RNA cDNA, using an equal volume of RNA for all samples.

Copyright and License information:  ©2022 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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