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Western blot

JZ Jun Zhang
YC Yongfei Cai
CL Christy L. Lavine
HP Hanqin Peng
HZ Haisun Zhu
KA Krishna Anand
PT Pei Tong
AG Avneesh Gautam
MM Megan L. Mayer
SR Sophia Rits-Volloch
SW Shaowei Wang
PS Piotr Sliz
DW Duane R. Wesemann
WY Wei Yang
MS Michael S. Seaman
JL Jianming Lu
TX Tianshu Xiao
BC Bing Chen
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Western blot was performed using an anti-SARS-COV-2 S antibody following a protocol described previously (Chen et al., 2015). Briefly, full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes. Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature. Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma-Aldrich, St. Louis, MO) was used as a secondary antibody. Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).

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