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Open field test

HS Hitomi Sasamori
TA Toshiaki Asakura
CS Chiaki Sugiura
YB Youcef Bouchekioua
NN Naoya Nishitani
MS Masaaki Sato
TY Takayuki Yoshida
MY Miwako Yamasaki
AT Akira Terao
MW Masahiko Watanabe
YO Yu Ohmura
MY Mitsuhiro Yoshioka
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A mouse was placed in an acrylic box (45 × 45 × 45 cm) for 70 min. The inside of the box was covered by rough-surfaced polypropylene sheets. The light intensity in the box was adjusted to 20 lx. The movement of each mouse was monitored through a CCD camera and was tracked using a software package (LimeLight, Actimetrics, USA). We considered the total distance traveled and the number of total crossings (defined by crossings of the lines made by the division of the chamber into 7.5 cm × 7.5 cm squares) as measures of locomotor activity. Moreover, we considered the percentage of time spent in the central area (15 cm × 15 cm square) as a measure of anxiety-like behavior.

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