Fecal Sample Collection

A total of 57 fecal samples were collected between December 2017 to March 2018, including 27 samples from children with allergic asthma, 10 samples from children with non-allergic asthma and 20 samples from healthy children. All participants, who offered feces, were asked to meet the following criteria, including no oral drug intake or no vaccination history in the past one month, no injury or diarrhea in the past two weeks, girls were not in the period of the menstrual cycle when offering feces. Fecal samples of all participants were collected into sterilized tubes and stored at a temperature of −80°C until detected. After removing low quality samples, gut microbiome was detected in 47 samples and metabolomics analysis was detected in 50 samples.