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Cell concentration and grouping resembled their counterparts in the above mentioned intracellular ROS assay. As mitochondrial membrane potential in RAW264.7 cells was determined via JC-1 fluorescent probe, these cells were cultivated by JC-1 working solution for 20 minutes at 37 °C after treated with LPS(1 μg/mL) for 12 h. Afterwards, JC-1 buffer liquor was applied to clean the cells three times at least. The outcomes were depicted by the rate of the green/red fluorescence strength, who referred to the level of mitochondrion disruption.
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