Statistical analysis

AA Asmaa Abbas
CW Colin W. Wright
NE Nagwa El-Sawi
TY Tapani Yli-Mattila
AM Anssi M. Malinen
request Request a Protocol
ask Ask a question
Favorite

Statistical analyses were performed using Origin (OriginLab) or SPSS Statistics 28 (IMB Corporation). Plant fraction content (TPC, TFC and antioxidants) and fungal growth and AFB1 production as a function of plant extract concentration data were analyzed using one-way analysis of variance (ANOVA) with post-hoc Tukey’s HSD. The null hypothesis—that observable means in any fraction or extract concentration are equal—was rejected where p < 0.01. Data normality was assumed.

RT-qPCR data were statistically tested in linearized form (i.e., as 2−(Ct, treated − Ct, control) values). The validity of the data normality assumption was confirmed by Shapiro–Wilk test, and the p-values of transcription level changes were calculated using a two-sided t-test. The criterion for statistical significance was set at a p-value < 0.05.

Search protocols in the Bio-protocol database

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A