In vivo deubiquitination assay

JT Jianing Tang
YL Yongwen Luo
LX Liang Xiao
ask Ask a question
Favorite

HEK293T cells were co-transfected with HA-Ub, Flag-TAZ, Myc-USP26, or Myc-USP26C304S plasmid as indicated using Lipofectamine 2000 for 24 h. After incubation with 10 μM MG132 (MCE) for 6 h, HA-ubiquitinated TAZ was immunoprecipitated with anti-Flag antibody followed by western blot analysis.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A