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Construction of the miRNA-mRNA Network

LW Li-Jun Wang
BQ Bai-Quan Qiu
MY Ming-Ming Yuan
HZ Hua-Xi Zou
CG Cheng-Wu Gong
HH Huang Huang
SL Song-Qing Lai
JL Ji-Chun Liu
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The differentially expressed miRNAs were uploaded to the miRWalk v2.0 database for predicting the potential targets of miRNAs (http://mirwalk.umm.uni-heidelberg.de/).16 Then, 39 mRNAs were obtained by taking the intersection between up-regulated miRNA targets (downregulated miRNA targets) and up-regulated DEMs (downregulated DEMs). To construct the initial miRNA-mRNA network, the miRNA-mRNA pairs were imported into Cytoscape software. All mRNAs were uploaded to the STRING database (https://string-db.org/), which was used to construct a protein-protein interaction network (PPI). The initial miRNA-mRNA and PPI network were merged into the final network.

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