Ethics statement

JK Jane Chanda Kabwe
HS Hirofumi Sawada
YM Yoshihide Mitani
HO Hironori Oshita
NT Naoki Tsuboya
EZ Erquan Zhang
JM Junko Maruyama
YM Yoshiki Miyasaka
HK Hideyoshi Ko
KO Kazunobu Oya
HI Hiromasa Ito
NY Noriko Yodoya
SO Shoichiro Otsuki
HO Hiroyuki Ohashi
RO Ryuji Okamoto
KD Kaoru Dohi
YN Yuhei Nishimura
TM Tomoji Mashimo
MH Masahiro Hirayama
KM Kazuo Maruyama
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Animal care, the experimental procedures, protocols for all animal experiments were approved by the Animal Research Ethics Committee, Mie University School of Medicine (Number 2019-28). All animal experiments were carried out in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publication). Catheterization and tissue harvest were performed under anesthesia using intraperitoneal sodium pentobarbital. Echocardiography was performed under anesthesia using isoflurane. During the course of the experiment, ethical endpoints were indicated based on feeding and watering difficulties, agonizing symptoms (e.g., dyspnea, self-injurious behaviour), prolonged external abnormalities (e.g., diarrhoea, bleeding) with no signs of recovery, and rapid weight loss (> 20% in a few days). All efforts were made to minimize pain perception in the animal studies.

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