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Maintenance in laboratory

FB Fedra Bollatti
CS Catalina Simian
AP Alfredo V. Peretti
AA Anita Aisenberg
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Individuals were maintained at the Laboratorio de Biología Reproductiva y Evolución of IDEA (UNC-CONICET), Argentina. Each individual was conditioned in 5 × 9 × 2 cm boxes, with sand from the original location as substrate and a wet cotton to provide water and moisture. Individuals were fed twice a week with larvae of Tenebrio sp. (Coleoptera, Tenebrionidae) and daily monitored to record molt occurence in order to determine the exact time at which individuals reached sexual maturity. After the experiments, voucher specimens were deposited at the Laboratorio de Biología Reproductiva y Evolución, LABRE-Ar, Instituto de Diversidad y Ecología Animal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina.

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