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BCa cells were transfected with biotinylated miRNA mimics or nonsense control (GenePharma, Shanghai, China) by lipofectamine 3000 (Invitrogen, Carlsbad, USA). The cells were harvested and lysed 24 h after transfection. Then, the cell lysates were incubated with streptavidin magnetic beads (Thermo Fisher Scientific, Shanghai, China) at 4 °C for 2 h. The beads were washed and the bound RNAs were extracted by RNeasy Mini Kit (QIAGEN, Shanghai, China). The abundance of hsa_circRNA_0088036 was analyzed by qRT-PCR analysis.
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