Primary human lung alveolar epithelial cells (Cell Biologics, H-6053) were seeded on 0.1% gelatin-coated 12-well plate according to manufacturer’s instruction. When reaching 70% confluent, cells were transfected with pCMV6-XL5 empty plasmid or S100A7 plasmid using TransIT-X2 Dynamic Delivery System (Mirus).
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