Culture and transfection of human primary alveolar epithelial cells

HB Haiqing Bai
LS Longlong Si
AJ Amanda Jiang
CB Chaitra Belgur
YZ Yunhao Zhai
RP Roberto Plebani
CO Crystal Yuri Oh
MR Melissa Rodas
AP Aditya Patil
AN Atiq Nurani
SG Sarah E. Gilpin
RP Rani K. Powers
GG Girija Goyal
RP Rachelle Prantil-Baun
DI Donald E. Ingber
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Primary human lung alveolar epithelial cells (Cell Biologics, H-6053) were seeded on 0.1% gelatin-coated 12-well plate according to manufacturer’s instruction. When reaching 70% confluent, cells were transfected with pCMV6-XL5 empty plasmid or S100A7 plasmid using TransIT-X2 Dynamic Delivery System (Mirus).

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