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Comparison of neutrophils before and after DH5α stimulation using flow cytometry
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The population of Cit-H3 positive cells in the NET area was determined to be more than 60% in the Side scatter and Forward scatter plots after DH5α stimulation. The expression levels of CD14, myeloperoxidase (MPO), and neutrophil elastase (NE) in neutrophils (untreated) and the NET area after 4 h of DH5α addition were compared by measuring the rate of fluorescence emitted from positive cells.
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