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Mouse generation and phenotyping

FN Fakhreddin Yaghoob Nezhad
AR Annett Riermeier
MS Martin Schönfelder
LB Lore Becker
MA Martin Hrabĕ de Angelis
HW Henning Wackerhage
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To study the role of the Hippo related genes in skeletal muscle, the hindlimbs of global knockout mice include Lats1−/− (Lats1em1(IMPC)H), Mst2−/− (Stk3em1(IMPC)H), Vgll3−/− (Vgll3em1(IMPC)H), and Vgll4+/− (Vgll4tm1b(EUCOMM)Hmgu) were kindly supplied by the Helmholtz Zentrum München (HMGU) in Germany, and the Medical Research Council Harwell (MRC Harwell) in the UK. Mice were generated on a C57BL/6 N background and phenotyping data were collected at the age of 16 weeks. For each of the Hippo-related genes knockout lines, at least three hind limbs of male mice were collected as well as age and sex-matched control mice of equivalent genetic backgrounds and sent to the Technical University of Munich for hindlimb muscle phenotyping. Information on the transgenesis, phenotyping, and data storage is available on the IMPC portal:

To phenotype the hindlimb muscles of Hippo gene-mutated mice and their matched control, we first dissected the hindlimb muscles of mutated and control mice. The analyzed muscles are the tibialis anterior (TA), extensor digitorum longus (EDL), gastrocnemius, and soleus. After dissection, we weighed the hindlimb muscles and then calculated the relative muscle weight (muscle weight/whole body weight).

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